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KMID : 0869420180160010013
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Journal of Jeahan Oriental Medical Academy
2018 Volume.16 No. 1 p.13 ~ p.23
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Anti-inflammatory effect of Hottuynia cordata root ethanol extract
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Jang Mi-Hee
Jung Dae-Hwa
Park Chung-A
Park Sook-Jahr
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Abstract
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Objectives：Hottuynia cordata is a traditional herb that has various physiological activity such as anti-cancer and anti-inflammation. We examined the anti-inflammatory effect of Hottuynia cordata root ethanol extract (HRE) in LPS-stimulated Raw264.7 cells.
Methods：Cell viability was measured by MTT assay. To detect nitric oxide (NO) production, culture media were mixed with Griess reagent and measured the absorbance at 540 nm. The levels of cytokines were determined with ELISA kit according to manufacturer's instructions. Western blot was applied to monitor protein expression levels.
Results：LPS-induced NO production was dose-dependently decreased by the treatment of HRE. The inhibitions of NO was due to the suppression of inducible nitric oxide synthase (iNOS) via nuclear factor-kappaB (NFκB), when determined by Western blot analysis. In addition, HRE efficiently inhibited pro-inflammatory cytokines such as TNF-α, IL-6 and IL-1β.
Conclusion：These results show that the root portion of Hottuynia cordata can be used as a herbal medicine with anti-inflammatory action.
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KEYWORD
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Hottuynia cordata, anti-inflammatory, LPS, Raw264.7 cell
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